Mouse P28 lung
5.08 um resolution
Fresh tissue
Ioversol contrast
Children's Hospital of Los Angeles
A series of 20X images stitched together of an E16.5 mouse lung. The right upper lobe (upper left), lower right lobe (bottom left), accessory lobe (bottom, middle) and left lobe (right) are depicted in this image.
H&E of lung from 20 month old human female; donation through NDRI
E16.5 mouse lung. The structure on the right is a bronchiole which is surrounded by alpha smooth muscle actin (Acta2, white). Within the smooth muscle is an artery with perivascular ACTA2 staining.
Video: Clarence Wigfall
Music: Simone Jackson Clark
Narrator: David Warburton
Graphics: Zhe Song
E16.5 mouse lung. Confocal imaging at 20X magnification showing protein expression of Nkx2.1 genes: TTF-1 (green, acinar tubules), Emcn (Endomucin, red, capillary bed), and Acta2 (Alpha smooth muscle actin, white, peribronchiolar and perivascular smooth muscle).
E16.5 mouse lung. An artery, surrounded by alpha smooth muscle actin (Acta2, white) is present on the left. Interspersed are endothelial cells expressing Endomucin (red) in the capillary bed and epithelial cells expressing NKX2.1 (green) in the acinar tubules.
Mouse P7 lung
3 um resolution
Dried tissue
No contrast
Children's Hospital of Los Angeles
E16.5 mouse lung showing protein expression of Nkx2.1 genes: TTF-1 (green, acinar tubules), Emcn (Endomucin, red, capillary bed), and Acta2 (Alpha smooth muscle actin, white, peribronchiolar and perivascular smooth muscle).
E16.5 mouse lung. NKX2.1 (TTF-1, green) is expressed in the acinar tubules
and Endomucin (EMCN, red) is expressed in the capillary bed.

Mouse E18.5 lung
5 um resolution
Fixed with sodium azide
OsO4 contrast
Children's Hospital of Los Angeles
E16.5 mouse lung. TTF1- is differentially expressed in the bronchiolar epithelium (weaker) and surrounding acinar tubules (stronger).

BREATH

BREATH is a GATEWAY to understanding perinatal lung maturation by integrating anatomic with molecular (omics) data.

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LungMAP

LungMAP seeks to improve lung health by providing the research community with a comprehensive web-based atlas to support investigations into the processes that regulate lung development. The use of cutting-edge technologies upon the many cell types from pre- and postnatal murine and human tissues will generate a novel map of where and when lung cells differentiate and alveoli form.